Studies on uroparotin. II. Alumina column chromatography on human and rabbit uroparotin.

It has been previously reported that the purified U. P. possessing remarkable Ca-reduction activity and circulating leucocyte effect in rabbit with dosage 1mg per kg is obtained by ethanol fractionation of the crude U. P. prepared from human urine by isoelectric precipitation. Further investigation led to the finding that Parotin-like substance exists in normal rabbit urine. This substance was extracted and purified by the same method as that of human U. P., and was found to have a twice stronger activity than human U. P. In order to obtain homogeneous substances with further purification of these U. P., column chromatography was tried. At first, the adsorption affinities of U. P. to various adsorbents, such as non-ionic, anionic,and cationic exchanger, were detected, and it was found that alumina was the most suitable one. Then, a remarkable purifying effect was observed with alumina column chromatography and highly purified U. P. could be obtained easily in good yield.